| Field | Specification |
|---|---|
| Target | |
| Alternative names | Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Cldn3; Cpetr2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Claudin 3/CLDN3, which shares 73.3% and 94.1% amino acid (aa) sequence identity with human and rat Claudin 3/CLDN3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets claudin-3 (gene Cldn3) in mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 23.3 kDa, and the supplier reports an observed band at about 23 kDa.
Claudin-3 (CLDN3) is a barrier-forming member of the claudin family that assembles into tight junction strands and seals the space between neighboring epithelial cells. It is expressed in many epithelia, and researchers use it as a marker of tight junction organization in studies of epithelial barrier function and cancer biology.
| Target | Claudin-3 (gene Cldn3; UniProt Q9Z0G9, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 23.3 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
Samples with a confirmed band (WB): rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:100, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): NIH/3T3 cells.
Immunogen
Synthetic peptide from the C-terminal region of mouse claudin 3/CLDN3. Sequence identity with the human and rat orthologs: 73.3% and 94.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places claudin-3 in the cell junction and cell membrane. Tissue expression noted by UniProt (mouse): Expressed in the lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huafeng Yang et al. (2024) Cornus Officinalis Total Glycosides Alleviate Granulomatous Lobular Mastitis via the B7-CD28/CTLA-4 Costimulatory Pathway. Chemistry & Biodiversity. 10.1002/cbdv.202401539
