| Field | Specification |
|---|---|
| Target | |
| Alternative names | Claudin-4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; CLDN4; CPER; CPETR1; WBSCR8 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Claudin 4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADEB-3 is a rabbit monoclonal antibody that recognizes claudin-4 (gene CLDN4) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 18 kDa.
Claudin-4 (CLDN4) is a tight junction protein that pairs with other claudins to build and remodel the strands sealing adjacent epithelial cells. Together with claudin-8 it can form anion-selective paracellular channels, including chloride pathways in the kidney, and its expression is frequently examined in studies of epithelial barriers and carcinomas.
| Target | Claudin-4 (gene CLDN4; UniProt O14493, human) |
|---|---|
| Clone | ADEB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human claudin 4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places claudin-4 in the cell junction and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Juan Wang et al. (2016) Mechanism of surface charge triggered intestinal epithelial tight junction opening upon chitosan nanoparticles for insulin oral delivery. Carbohydrate Polymers. 10.1016/j.carbpol.2016.10.021
