Anti-Claudin 5/CLDN5 Antibody

Overview

Anti-Claudin 5/CLDN5 Antibody is an antibody for CLDN5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CLDN5 (thrombospondin 2); UniProt: O00501
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 130 kDa
• Applications: WB, IHC, IF, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Claudin 5/CLDN5 Antibody catalog # A03260-3.

Biological background

Biological context: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.

Expression and localization notes: cellular localization: Synapse. Postsynaptic density. Dendritic spine. Dendrite., tissue context: High expression in invertebral disk tissue..

Common research applications

• Western blotting (WB): Compare CLDN5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CLDN5 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify CLDN5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Synapse. Postsynaptic density. Dendritic spine. Dendrite.
• Tissue details: High expression in invertebral disk tissue.
• Research category: Calcium Signaling,Calmodulin Pathway,Cardiovascular,Hypertrophy,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.