| Field | Specification |
|---|---|
| Target | |
| Alternative names | Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF; CLDN5; AWAL; TMVCF |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5/CLDN5, which shares 95% and 90% amino acid (aa) sequence identity with mouse and rat Claudin 5/CLDN5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects claudin-5 (gene CLDN5) in human samples and is validated for IHC and ICC/IF. The predicted molecular weight is 23.1 kDa.
Claudin-5 (CLDN5) is the main claudin of endothelial tight junctions and is especially abundant in brain capillaries. By sealing the gaps between endothelial cells it is a key determinant of blood–brain barrier tightness, so it is a standard marker in studies of vascular permeability and neurovascular biology.
| Target | Claudin-5 (gene CLDN5; UniProt O00501, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 23.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 1–2 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Immunogen
Synthetic peptide from the C-terminal region of human claudin 5/CLDN5. Sequence identity with the mouse and rat orthologs: 95% and 90%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places claudin-5 in the cell junction and cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tian Jijing et al. (2019) Brain Infection by Hepatitis E Virus Probably via Damage of the Blood-Brain Barrier Due to Alterations of Tight Junction Proteins. Frontiers in Cellular and Infection Microbiology. 10.3389/fcimb.2019.00052
