Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody

Overview

This product is an anti-CLDN5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAHA-3; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Claudin 5 CLDN5 Rabbit Monoclonal Antibody catalog # M03260. Tested in WB, IHC applications. This antibody reacts with Human.

Key elements and design rationale

• Target: CLDN5 (Claudin-5).
• Antibody format: Monoclonal; clone AAHA-3; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

CLDN5 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Plays a major role in tight junction-specific obliteration of the intercellular space. . Reported cellular localization context: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Highly expressed in brain. Expressed in many tissues. .

Research relevance and current trends

• Research context keywords from the source record include: Cell Adhesion,Cytoskeleton/ECM,Signal Transduction,Tight Junctions.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.

Workflow ideas (metafield): Validate CLDN5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CLDN5 expression by Western blot in cell or tissue lysates, Detect CLDN5 in FFPE tissue sections by immunohistochemistry

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa; calculated MW: 23147 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 130 kDa
• Cellular localization (provided): Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
• Tissue details (provided): Highly expressed in brain. Expressed in many tissues. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.