| Field | Specification |
|---|---|
| Target | |
| Alternative names | Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Claudin 5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAHA-3 is a rabbit monoclonal antibody that recognizes claudin-5 (gene CLDN5) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 23.1 kDa, and the supplier reports an observed band at about 20 kDa.
Claudin-5 (CLDN5) is the main claudin of endothelial tight junctions and is especially abundant in brain capillaries. By sealing the gaps between endothelial cells it is a key determinant of blood–brain barrier tightness, so it is a standard marker in studies of vascular permeability and neurovascular biology.
| Target | Claudin-5 (gene CLDN5; UniProt O00501, human) |
|---|---|
| Clone | AAHA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.1 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human claudin 5.
Reactivity Notes
The supplier lists reactivity with human. UniProt places claudin-5 in the cell junction and cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
