| Field | Specification |
|---|---|
| Target | |
| Alternative names | H; +; /Cl; -; exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3; CLCN3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CLCN3 recombinant protein (Position: G293-R752). Human CLCN3 shares 99.6% amino acid (aa) sequence identity with mouse CLCN3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects H(+)/Cl(-) exchange transporter 3 (gene CLCN3) in human and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
ClC-3 (CLCN3) is a member of the CLC family that works as an electrogenic 2Cl−/H+ exchanger in endosomes and synaptic vesicles. It supports acidification and chloride accumulation in these compartments, and it is studied in neuronal function and endosomal trafficking.
| Target | H(+)/Cl(-) exchange transporter 3 (gene CLCN3; UniProt P51790, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CLCN3 fragment (Gly293–Arg752), expressed in E. coli. Sequence identity with the mouse ortholog: 99.6%.
Reactivity Notes
The supplier lists reactivity with human and rat. Tissue expression noted by UniProt (human): Expressed primarily in tissues derived from neuroectoderm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lijuan Xu et al. (2013) ClC-3 chloride channel in hippocampal neuronal apoptosis. Neural Regeneration Research. 10.3969/j.issn.1673-5374.2013.32.008
