| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caspase-9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEE-3 is a rabbit monoclonal antibody that recognizes caspase-9 (gene CASP9) in human and mouse samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 46.3 kDa, and the supplier reports an observed band at about 46 kDa.
Caspase-9 is the initiator caspase of the intrinsic, mitochondrial apoptosis pathway. When cytochrome c is released from mitochondria, it binds Apaf-1 to form the apoptosome, which activates caspase-9; caspase-9 then cleaves and activates caspase-3 and caspase-7. It is a standard marker in studies of DNA damage-induced and stress-induced apoptosis.
| Target | Caspase-9 (gene CASP9; UniProt P55211, human) |
|---|---|
| Clone | FEE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.3 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human caspase-9.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chengquan Han et al. (2025) Integrated network pharmacology, molecular docking and in vivo experiments to elucidate the extenuative mechanisms of ginseng polysaccharide against Toxoplasma gondii-induced testicular toxicity. International Immunopharmacology. 10.1016/j.intimp.2025.114147
Hanyue Zheng et al. (2024) Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study. Nanomaterials. 10.3390/nano14080689
Yanfei He et al. (2024) Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment. Journal of Functional Foods. 10.1016/j.jff.2024.106314
Fenglin Sun et al. (2019) Acacetin-induced cell apoptosis in head and neck squamous cell carcinoma cells: Evidence for the role of muscarinic M3 receptor. Phytotherapy Research. 10.1002/ptr.6343
Ozgecan Kayalar et al. (2024) Retinoic acid reduces kidney injury by regulating oxidative stress, NRF-2, and apoptosis in hyperoxic mice. Cell Biochemistry and Function. 10.1002/cbf.4094
Chen Zhao et al. (2018) Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress. Gastroenterology Research and Practice. 10.1155/2018/9252984
Li Bing et al. (2009) Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin. ACTA Biochimica et Biophysica Sinica. 10.1093/abbs/gmp104
