| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chloride intracellular channel protein 1; Chloride channel ABP; Glutaredoxin-like oxidoreductase CLIC1; Glutathione-dependent dehydroascorbate reductase CLIC1; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC; CLIC1; G6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CLIC1 recombinant protein (Position: D47-K241). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects chloride intracellular channel protein 1 (gene CLIC1) in human, rat and monkey samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 26.9 kDa, and the supplier reports an observed band at about 32 kDa.
CLIC1 is a member of the chloride intracellular channel family that exists both as a soluble protein and in a membrane-inserted form. In solution it shows glutaredoxin-like thiol oxidoreductase activity, while in membranes it can form ion channels, and it is studied in oxidative stress, cell proliferation and tumor biology.
| Target | Chloride intracellular channel protein 1 (gene CLIC1; UniProt O00299, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.9 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat, Monkey) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HL-60 cells, human HeLa cells, monkey COS-7 cells, human THP-1 cells, human Caco-2 cells, human U-937 cells, rat kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human CLIC1 fragment (Asp47–Lys241), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, rat and monkey. UniProt places chloride intracellular channel protein 1 in the nucleus, nuclear membrane, cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expression is prominent in heart, placenta, liver, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
