| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent Clp protease proteolytic subunit, mitochondrial; Caseinolytic mitochondrial matrix peptidase proteolytic subunit; Endopeptidase Clp; CLPP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CLPP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24C55 is a rabbit monoclonal antibody that recognizes ATP-dependent Clp protease proteolytic subunit, mitochondrial (gene CLPP) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 30.2 kDa, and the supplier reports an observed band at about 26 kDa.
CLPP is the proteolytic subunit of the mitochondrial ClpXP protease. Working with the unfoldase CLPX, it degrades misfolded or damaged proteins in the mitochondrial matrix and contributes to mitochondrial protein quality control, so it is studied in mitochondrial stress responses and as a target of small-molecule activators.
| Target | ATP-dependent Clp protease proteolytic subunit, mitochondrial (gene CLPP; UniProt Q16740, human) |
|---|---|
| Clone | 24C55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.2 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CLPP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent Clp protease proteolytic subunit, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Detected in liver (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
