| Field | Specification |
|---|---|
| Target | |
| Alternative names | Putative lipid scramblase CLPTM1; Cleft lip and palate transmembrane protein 1; CLPTM1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CLPTM1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27C56 is a rabbit monoclonal antibody that recognizes putative lipid scramblase CLPTM1 in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 76.1 kDa, and the supplier reports an observed band at about 90 kDa.
CLPTM1 is a multi-pass membrane protein of the endoplasmic reticulum proposed to act as a lipid scramblase. In neurons it can bind GABAA receptors and retain them in the ER, reducing their surface level and inhibitory neurotransmission, so it is studied in synaptic receptor trafficking.
| Target | Putative lipid scramblase CLPTM1 (gene CLPTM1; UniProt O96005, human) |
|---|---|
| Clone | 27C56 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.1 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CLPTM1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places putative lipid scramblase CLPTM1 in the membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
