| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calsyntenin-1; Alcadein-alpha; Alc-alpha; Alzheimer-related cadherin-like protein; Non-classical cadherin XB31alpha; CLSTN1; CS1; KIAA0911 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CLSTN1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21C89 is a rabbit monoclonal antibody that recognizes calsyntenin-1 (gene CLSTN1) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 109.8 kDa, and the supplier reports an observed band at about 150 kDa.
Calsyntenin-1 (CLSTN1) is a transmembrane cadherin-superfamily protein enriched in neurons. It acts as a synaptic adhesion molecule that binds presynaptic neurexins to support synapse formation, and it also serves as a kinesin-1 cargo adaptor in axonal transport, so it is studied in synapse development and neuronal trafficking.
| Target | Calsyntenin-1 (gene CLSTN1; UniProt O94985, human) |
|---|---|
| Clone | 21C89 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 109.8 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human CLSTN1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places calsyntenin-1 in the postsynaptic cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane and cell projection. Tissue expression noted by UniProt (human): Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
