| Field | Specification |
|---|---|
| Target | |
| Alternative names | Clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Sulfated glycoprotein 2; SGP-2; Testosterone-repressed prostate message 2; TRPM-2; CLU; APOJ; KUB1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Clusterin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17C92 is a rabbit monoclonal antibody that recognizes clusterin in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 52.5 kDa, and the supplier reports an observed band at about 65 kDa.
Clusterin (CLU, also called apolipoprotein J) is a secreted glycoprotein that works as an extracellular chaperone, binding misfolded proteins and preventing their aggregation, including amyloid-forming proteins. It is abundant in plasma and many body fluids, and it is widely studied in protein homeostasis, lipid transport, neurodegeneration and cell survival.
| Target | Clusterin (gene CLU; UniProt P10909, human) |
|---|---|
| Clone | 17C92 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.5 kDa |
| Observed MW | 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human U2OS cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human clusterin.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Z.Z. Yin et al. (2006) Sertoli Cells Induce Xenolymphocyte Apoptosis In Vitro. Transplantation Proceedings. 10.1016/j.transproceed.2006.10.126
