| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 2',3'-cyclic-nucleotide 3'-phosphodiesterase;CNP;CNPase;3.1.4.37;CNP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human CNPase, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CNP (2',3'-cyclic-nucleotide 3'-phosphodiesterase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CNPase Antibody Picoband® catalog # A01017. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human CNPase, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
- Molecular weight context: reported MW: 47 kDa; calculated MW: 47579 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
2',3'-cyclic-nucleotide 3'-phosphodiesterase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase. 2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles. Functional note: May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. . Reported localization: Membrane; Lipid-anchor. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Research relevance and current trends
- Cell Type Marker: Researchers commonly examine how CNP (2',3'-cyclic-nucleotide 3'-phosphodiesterase) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how CNP (2',3'-cyclic-nucleotide 3'-phosphodiesterase) relates to this theme using model systems and orthogonal readouts.
- Neurodegenerative Disease: Researchers commonly examine how CNP (2',3'-cyclic-nucleotide 3'-phosphodiesterase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CNP (2',3'-cyclic-nucleotide 3'-phosphodiesterase) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
