| Field | Specification |
|---|---|
| Target | |
| Alternative names | Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; CNTN4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CNTN4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27C46 is a rabbit monoclonal antibody that recognizes contactin-4 (gene CNTN4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 113.5 kDa, and the supplier reports an observed band at about 150 kDa.
Contactin-4 (CNTN4) is a GPI-anchored neural adhesion molecule of the contactin family. It promotes neurite outgrowth and has been implicated in axon targeting and synapse formation, and it is studied in neural circuit development.
| Target | Contactin-4 (gene CNTN4; UniProt Q8IWV2, human) |
|---|---|
| Clone | 27C46 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 113.5 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human cerebellum cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CNTN4.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes contactin-4 as secreted and also located in the cell membrane. Tissue expression noted by UniProt (human): Mainly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
