Anti-COL11A1 Rabbit Monoclonal Antibody

SKU:BHA21010101
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-COL11A1 antibody (Rabbit monoclonal, clone 26C08, IgG). Validated for Western blot. Reactivity: Human. Recognizes collagen alpha-1(XI) chain. Observed band in Western blot: about 150 kDa.
Target COL11A1
clone number 26C08
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M02909 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target COL11A1
Alternative names Collagen alpha-1; XI; chain; COL11A1; COLL6
Gene ID 1301
UniProt # P12107
Host Rabbit
Clone 26C08
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human COL11A1
Molecular weight 181.1 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted, extracellular space, extracellular matrix.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M02909
Main SKU BHA21010101
Rabbit Monoclonal

Product Overview

Clone 26C08 is a rabbit monoclonal antibody that recognizes collagen alpha-1(XI) chain (gene COL11A1) in human samples and is validated for Western blot. The predicted molecular weight is 181.1 kDa, and the supplier reports an observed band at about 150 kDa.

Collagen α1(XI) chain (COL11A1) is a component of type XI collagen, a minor fibrillar collagen of cartilage. It co-assembles with type II collagen and helps regulate fibril diameter, and it is studied in skeletal development, hearing and eye research, and in stromal changes in cancer.

Target Collagen alpha-1(XI) chain (gene COL11A1; UniProt P12107, human)
Clone 26C08
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 181.1 kDa
Observed MW 150 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): K562 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human COL11A1.

Reactivity Notes

The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Cartilage, placenta and some tumor or virally transformed cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 181.1 kDa; observed band at about 150 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human COL11A1.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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