| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (I) chain;Alpha-1 type I collagen;COL1A1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen I, different from the related mouse and rat sequences by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes collagen alpha-1(I) chain (gene COL1A1) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 138.9 kDa, and the supplier reports an observed band at about 130–180 kDa.
Collagen α1(I) chain (COL1A1) is the major chain of type I collagen, the most abundant fibrillar collagen in bone, skin, tendon and other connective tissues. It is a standard marker of fibroblasts, osteoblasts and fibrosis, and COL1A1 mutations cause osteogenesis imperfecta.
| Target | Collagen alpha-1(I) chain (gene COL1A1; UniProt P02452, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 138.9 kDa |
| Observed MW | 130–180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human placenta tissue, rat skin tissue, rat lung tissue, mouse skin tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human placenta tissue, human renal cells carcinoma tissue, human bladder urothelial carcinoma tissue, human lung adenocarcinoma tissue, human cervical cancer tissue, human tonsillitis tissue, human appendicitis tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human placenta tissue, mouse lung tissue, rat lung tissue.
Immunogen
Synthetic peptide from the C-terminal region of human collagen I. It differs from the mouse and rat sequences at four residues.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Forms the fibrils of tendon, ligaments and bones. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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