| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (I) chain;Alpha-1 type I collagen;Col1a1;Cola1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I, different from the related human sequence by three amino acids, and identical to the related rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects collagen alpha-1(I) chain (gene Col1a1) in mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 138.0 kDa; the supplier reports observed bands at 130,180–200 kDa.
Collagen α1(I) chain (COL1A1) is the major chain of type I collagen, the most abundant fibrillar collagen in bone, skin, tendon and other connective tissues. It is a standard marker of fibroblasts, osteoblasts and fibrosis, and COL1A1 mutations cause osteogenesis imperfecta.
| Target | Collagen alpha-1(I) chain (gene Col1a1; UniProt P11087, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 138.0 kDa |
| Observed MW | 130,180–200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat lung tissue, rat skin tissue, mouse lung tissue, mouse skin tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of mouse collagen I. The peptide sequence is conserved in rat. It differs from the human sequence at three residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. Tissue expression noted by UniProt (mouse): Forms the fibrils of tendon, ligaments and bones. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yongyuan Kang et al. (2025) Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation. Advanced Functional Materials. 10.1002/adfm.202413678
Pingping Niu et al. (2024) Self-Assembled Nanoparticles with Well-Defined Oligosaccharide Promote Osteogenesis by Regulating Golgi Stress Response. Advanced Healthcare Materials. 10.1002/adhm.202402976
Shuaiqiang Zhang et al. (2018) Prostaglandin E2 hydrogel improves cutaneous wound healing via M2 macrophages polarization. Theranostics. 10.7150/thno.27385
Wentao Shi et al. (2022) Alendronate crosslinked chitosan/polycaprolactone scaffold for bone defects repairing. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2022.02.007
Yiqun Zhang et al. (2023) 3D-printed scaffolds of mesoporous bioglass/gliadin/polycaprolactone ternary composite for enhancement of compressive strength, degradability, cell responses and new bone tissue ingrowth. International Journal of Nanomedicine. 10.2147/IJN.S164869
Meng Li et al. (2024) A heparin-functionalized bioink with sustained delivery of vascular endothelial growth factor for 3D bioprinting of prevascularized dermal constructs. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.130075
Shihe Long et al. (2022) A sonication-induced silk-collagen hydrogel for functional cartilage regeneration. Journal of Materials Chemistry B. 10.1039/D2TB00564F
Meng Li et al. (2023) 3D bioprinting of heterogeneous tissue-engineered skin containing human dermal fibroblasts and keratinocytes. Biomaterials Science. 10.1039/D2BM02092K
Ye He et al. (2018) Peptide LL-37 coating on micro-structured titanium implants to facilitate bone formation in vivo via mesenchymal stem cell recruitment. Acta Biomaterialia. 10.1016/j.actbio.2018.09.036
Xiaoqin Zhang et al. (2022) Chimonanthus salicifolius attenuated vascular remodeling by alleviating endoplasmic reticulum stress in spontaneously hypertensive rats. Food & Function. 10.1039/D1FO04381A
