| Field | Specification |
|---|---|
| Target | |
| Alternative names | COL1A1; Collagen alpha-1(I chain; Alpha-1 type I collagen |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Collagen I |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFD-3 is a rabbit monoclonal antibody that recognizes COL1A1 in human samples and is validated for Western blot and IHC.
| Target | COL1A1 (UniProt P02452/P08123) |
|---|---|
| Clone | AFD-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 139 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast cancer tissue, human colorectal adenocarcinoma tissue, human lung squamous cells carcinoma tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human collagen I.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Lai I-Tzu et al. (2024) Investigating the role of various extracellular matrix in androgenetic alopecia: Insights from immunostaining and quantitative analysis – A pilot study. Dermatologica Sinica. 10.4103/ds.DS-D-23-00210
Zhenhui Lu et al. (2014) A novel synthesized sulfonamido-based gallic acid – LDQN-C: Effects on chondrocytes growth and phenotype maintenance. Bioorganic Chemistry. 10.1016/j.bioorg.2014.09.005
Hui Tao et al. (2017) MicroRNA-21 via Dysregulation of WW Domain-Containing Protein 1 Regulate Atrial Fibrosis in Atrial Fibrillation. Heart Lung and Circulation. 10.1016/j.hlc.2016.01.022
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