| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (II) chain; Alpha-1 type II collagen; Collagen alpha-1 (II) chain; Chondrocalcin |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, which shares 91.7% and 95.8% amino acid (aa) sequence identity with mouse and rat Collagen II, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects collagen alpha-1(II) chain (gene COL2A1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 141.8 kDa, and the supplier reports an observed band at about 180–190 kDa.
Collagen α1(II) chain (COL2A1) forms type II collagen, the main collagen of hyaline cartilage and the vitreous of the eye. It is essential for skeletal development and for cartilage resistance to compression, and it is a standard chondrocyte marker in cartilage and osteoarthritis research.
| Target | Collagen alpha-1(II) chain (gene COL2A1; UniProt P02458, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 141.8 kDa |
| Observed MW | 180–190 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): rat cartilage tissue, mouse cartilage tissue, mouse auricular cartilage.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human collagen II. Sequence identity with the mouse and rat orthologs: 91.7% and 95.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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