| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (II) chain;Alpha-1 type II collagen;Collagen alpha-1 (II) chain;Chondrocalcin;Col2a1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes collagen alpha-1(II) chain (gene Col2a1) in mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 142.0 kDa, and the supplier reports an observed band at about 170 kDa.
Collagen α1(II) chain (COL2A1) forms type II collagen, the main collagen of hyaline cartilage and the vitreous of the eye. It is essential for skeletal development and for cartilage resistance to compression, and it is a standard chondrocyte marker in cartilage and osteoarthritis research.
| Target | Collagen alpha-1(II) chain (gene Col2a1; UniProt P28481, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 142.0 kDa |
| Observed MW | 170 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Mouse) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Rat, Mouse) |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Samples with a confirmed band (WB): rat trachea tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (ICC/IF): mouse cartilage tissue.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of mouse collagen II1222–1241aa AFAGLGQREKGPDPMQYMRA). It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiaoling Zhu et al. (2025) Procr+ chondroprogenitors sense mechanical stimuli to govern articular cartilage maintenance and regeneration. Cell. 10.1016/j.cell.2025.06.036
Luo Ya-Biao et al. (2025) Telomere-to-telomere genome assembly of a male pig provides insight into population structure and selection for body stature. Nature Genetics. 10.1038/s41588-025-02433-6
Jiang Bo et al. (2026) Fibrous-layer resident Angptl7+ periosteal stem cells sense injury inflammation to orchestrate fracture repair. Cell Research. 10.1038/s41422-025-01202-8
Yushan Wang et al. (2026) NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties. Bioactive Materials. 10.1016/j.bioactmat.2026.01.006
Junchao Huang et al. (2026) Iron Sequestration by Magnesium Prussian Blue to Ameliorate Osteoarthritis Through Restoration of HIF-1α Signaling and Ferroptosis Inhibition. Advanced Functional Materials. 10.1002/adfm.76099
Yuxiang Fei et al. (2024) Promoting chondrogenesis by targeted delivery to the degenerating cartilage in early treatment of osteoarthritis. Bioactive Materials. 10.1016/j.bioactmat.2024.08.004
Yu Qin et al. (2021) A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real-Time Monitoring of Cells. Advanced Science. 10.1002/advs.202003738
Jie Lv et al. (2025) Defective RuO2 Nanospheres Attenuate Osteoarthritis Progression via Suppressing the ROS/NLRP3/Caspase-1 Signaling Pathway. ACS Nano. 10.1021/acsnano.5c04011
Suo Jinlong et al. (2025) Targeting VGLL4 maintains extracellular matrix homeostasis and mitigates osteoarthritis in a preclinical model. Nature Communications. 10.1038/s41467-025-64361-7
Xu Jiaojiao et al. (2025) CD133+PD-L1+ cancer cells confer resistance to adoptively transferred engineered macrophage-based therapy in melanoma. Nature Communications. 10.1038/s41467-025-55876-0
