| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (III) chain; COL3A1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes collagen alpha-1(III) chain (gene COL3A1) in human, mouse and rat samples and is validated for ELISA, flow cytometry, IHC and Western blot. The predicted molecular weight is 138.6 kDa, and the supplier reports an observed band at about 200 kDa.
Collagen α1(III) chain (COL3A1) forms type III collagen, a fibrillar collagen that occurs with type I collagen in skin, blood vessels and hollow organs. It supports tissue elasticity and strength, and COL3A1 mutations cause vascular Ehlers-Danlos syndrome; it is also studied in wound healing and fibrosis.
| Target | Collagen alpha-1(III) chain (gene COL3A1; UniProt P02461, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 138.6 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse skin tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gastric poorly differentiated adenocarcinoma tissue, human tonsil tissue, human liver cancer tissue, human lung adenocarcinoma tissue, mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human Collagen III/COL3A1 fragment (Asp1222–Glu1455), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yijie Fang et al. (2024) Collagen denaturation in post-run Achilles tendons and Achilles tendinopathy: In vivo mechanophysiology and magnetic resonance imaging. Science Advances. 10.1126/sciadv.ado2015
Zhong Lingzhi et al. (2025) Forskolin-loaded carboxymethyl chitosan and silk nonofiber hydrogels composite scaffolds improve skin regeneration. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.142931
Huiming Jiang et al. (2026) Methylene blue restores NAD+/NADH homeostasis to attenuate Achilles tendinopathy via activating AIF/Mitochondrial respiratory chain complex I. Journal of Orthopaedic Translation. 10.1016/j.jot.2026.101085
Ling Li et al. (2022) Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition. Oxidative Medicine and Cellular Longevity. 10.1155/2022/7434733
Dali Ding et al. (2023) Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis. Phytotherapy Research. 10.1002/ptr.7774
Deshuang Meng et al. (2024) Exosomes Derived from Antler Mesenchymal Stem Cells Promote Wound Healing by miR-21-5p/STAT3 Axis. International Journal of Nanomedicine. 10.2147/IJN.S481044
Luo Hong et al. (2023) Salvianolic acid B ameliorates myocardial fibrosis in diabetic cardiomyopathy by deubiquitinating Smad7. Chinese Medicine. 10.1186/s13020-023-00868-9
Yanwei Cao et al. (2022) Platelet-rich plasma pretreatment protects anterior cruciate ligament fibroblasts correlated with PI3K-Akt-mTOR pathway under hypoxia condition. Journal of Orthopaedic Translation. 10.1016/j.jot.2022.02.002
Ting Liu et al. (2024) FAP+ gastric cancer mesenchymal stromal cells via paracrining INHBA and remodeling ECM promote tumor progression. International Immunopharmacology. 10.1016/j.intimp.2024.113697
Yue Qin et al. (2016) Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway. International Journal of Nanomedicine. 10.2147/IJN.S123839
