| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1; III; chain; COL3A1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from Collagen III |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26C74 is a rabbit monoclonal antibody that recognizes collagen alpha-1(III) chain (gene COL3A1) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 138.6 kDa, and the supplier reports an observed band at about 150 kDa-200 kDa.
Collagen α1(III) chain (COL3A1) forms type III collagen, a fibrillar collagen that occurs with type I collagen in skin, blood vessels and hollow organs. It supports tissue elasticity and strength, and COL3A1 mutations cause vascular Ehlers-Danlos syndrome; it is also studied in wound healing and fibrosis.
| Target | Collagen alpha-1(III) chain (gene COL3A1; UniProt P02461, human) |
|---|---|
| Clone | 26C74 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 138.6 kDa |
| Observed MW | 150 kDa-200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of collagen III.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Wang Qing et al. (2019) Glycemic control is associated with atrial structural remodeling in patients with type 2 diabetes. BMC Cardiovascular Disorders. 10.1186/s12872-019-1249-2
Wen-Song Ge et al. (2014) β-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/β-catenin signaling inhibits hepatic stellate cell activation. Molecular Medicine Reports. 10.3892/mmr.2014.2099
Zhen-Chang Wang et al. (2014) Effect of Rougan Huaqian granules combined with human mesenchymal stem cell transplantation on liver fibrosis in cirrhosis rats. Asian Pacific Journal of Tropical Medicine. 10.1016/S1995-7645(14)60097-3
