| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1; II; chain; Alpha-1 type II collagen; COL2A1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets collagen alpha-1(II) chain (gene COL2A1) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA.
Collagen α1(II) chain (COL2A1) forms type II collagen, the main collagen of hyaline cartilage and the vitreous of the eye. It is essential for skeletal development and for cartilage resistance to compression, and it is a standard chondrocyte marker in cartilage and osteoarthritis research.
| Target | Collagen alpha-1(II) chain (gene COL2A1; UniProt P02458, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Collagen Type II/COL2A1 fragment (Gly1217–Ala1241), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chenggong Ma et al. (2025) WYRGRL-functionalized tetrahedral DNA integrates ROS scavenging and miR-26b-5p delivery for cartilage-specific osteoarthritis treatment. Chemical Engineering Journal. 10.1016/j.cej.2025.170673
Dongyang Li et al. (2026) Cyanidin-3-O-glucoside chloride inhibits cartilage degeneration and inflammation in TMJOA via YAP/NF-κB signaling pathway. Food & Function. 10.1039/D5FO04230E
Dongmei Yan et al. (2026) Platelet-Rich Plasma Attenuates Knee Osteoarthritis in Rats via Modulation of Gut Microbiota. Drug Design Development and Therapy. 10.2147/DDDT.S574392
Xueyuan Sun et al. (2026) Platelet-rich plasma ameliorates knee osteoarthritis in rats by modulating the abundance of gut Lactobacillus murinus. Journal of Drug Targeting. 10.1080/1061186X.2026.2648088
Ludan Xing et al. (2024) Histopathological Characterization of Mandibular Condyles in Four Temporomandibular Joint Osteoarthritis Mouse Models. Archives of Oral Biology. 10.1016/j.archoralbio.2024.106129
