| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (X) chain;COL10A1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Collagen X |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOAH-3 is a rabbit monoclonal antibody that recognizes collagen alpha-1(X) chain (gene COL10A1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 66.2 kDa, and the supplier reports an observed band at about 66 kDa.
Type X collagen (COL10A1) is a short-chain collagen made by hypertrophic chondrocytes in the growth plate. It is localized to regions of cartilage that are about to mineralize during endochondral bone formation, so it is a standard marker of chondrocyte hypertrophy in skeletal development and osteoarthritis studies.
| Target | Collagen alpha-1(X) chain (gene COL10A1; UniProt Q03692, human) |
|---|---|
| Clone | AOAH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 66.2 kDa |
| Observed MW | 66 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): mouse liver tissue, rat liver tissue, human fetal skin.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human collagen X.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Zhu et al. (2022) A Photoannealed Granular Hydrogel Facilitating Hyaline Cartilage Regeneration via Improving Chondrogenic Phenotype. ACS Applied Materials & Interfaces. 10.1021/acsami.2c11956
Yongli Gao et al. (2024) Collagen-based hydrogels induce hyaline cartilage regeneration by immunomodulation and homeostasis maintenance. Acta Biomaterialia. 10.1016/j.actbio.2024.07.018
Tongyao Yu et al. (2025) Rotating magnetic field downregulating type XI collagen to suppress triple-negative breast cancer metastasis by inactivating the ITGB1/FAK/YAP signaling pathway. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.149677
Qianqian Duan et al. (2026) CDs-PEI/siIhh Delivery System for the Treatment of Osteoarthritis. ACS Applied Bio Materials. 10.1021/acsabm.5c02517
Wang Wenchao et al. (2024) TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis. Arthritis Research & Therapy. 10.1186/s13075-024-03304-x
