| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (XVII) chain;180 kDa bullous pemphigoid antigen 2;Bullous pemphigoid antigen 2;120 kDa linear IgA disease antigen;120 kDa linear IgA dermatosis antigen;Linear IgA disease antigen 1;LAD-1;97 kDa linear IgA disease antigen;97 kDa linear IgA bullous dermatosis antigen;97 kDa LAD antigen;97-LAD;Linear IgA bullous disease antigen of 97 kDa;LABD97;COL17A1;BP180, BPAG2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Collagen XVII |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AA-3 is a rabbit monoclonal antibody that recognizes collagen alpha-1(XVII) chain (gene COL17A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 150.4 kDa; the supplier reports observed bands at 130, 180 kDa.
Collagen XVII (COL17A1, also known as BP180) is a transmembrane collagen of hemidesmosomes that anchors basal keratinocytes to the underlying basement membrane. It is an autoantigen in bullous pemphigoid, and its shed ectodomain is a target in linear IgA disease, making it central to skin adhesion research.
| Target | Collagen alpha-1(XVII) chain (gene COL17A1; UniProt Q9UMD9, human) |
|---|---|
| Clone | AA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 150.4 kDa |
| Observed MW | 130, 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with confirmed staining (IHC): mouse skin tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide based on the sequence of human collagen XVII.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places collagen alpha-1(XVII) chain in the cell junction and membrane. Tissue expression noted by UniProt (human): Detected in skin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhe Xu et al. (2023) Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.17833
Ning Cheng et al. (2013) The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation. PLoS One. 10.1371/journal.pone.0081014
