| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement component C9;Complement component C9a;Complement component C9b;C9; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Complement C9 recombinant protein (Position: K289-N515). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects complement component C9 in human and mouse samples and is validated for ELISA and Western blot. The predicted molecular weight is 63.2 kDa, and the supplier reports an observed band at about 80 kDa.
Complement C9 is the final, pore-forming protein of the membrane attack complex. Recruited by C5b-8, multiple C9 molecules polymerize into a ring that punches a pore through the target membrane and lyses bacteria or cells. It is studied in innate immunity against Gram-negative bacteria and in complement-mediated tissue damage.
| Target | Complement component C9 (gene C9; UniProt P02748, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 63.2 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, mouse liver tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Complement C9 fragment (Lys289–Asn515), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes complement component C9 as secreted and also located in the target cell membrane. Tissue expression noted by UniProt (human): Plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Daniel E. Venegas-Pino et al. (2018) Evidence of extensive atherosclerosis, coronary artery disease and myocardial infarction in the ApoE−/−:Ins2+/Akita mouse fed a western diet. Atherosclerosis. 10.1016/j.atherosclerosis.2018.05.044
Xin Wang et al. (2020) Complement factor B knockdown by short hairpin RNA inhibits laser-induced choroidal neovascularization in rats. International Journal of Ophthalmology. 10.18240/ijo.2020.03.03
