| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Neural cell surface protein F3, Glycoprotein GP135, CNTN1 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Contactin-1/CNTN1 (extracellular) Antibody is an antibody targeting Neural cell surface protein F3, Glycoprotein GP135, CNTN1 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Neural cell surface protein F3, Glycoprotein GP135, CNTN1 (also reported as Neural cell surface protein F3, Glycoprotein GP135, CNTN1).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Mouse - identical; human - 14/15 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Cited use: IHC (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Contactin-1 (CNTN1), also known as F3/Contactin a cell adhesion molecule, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein involved in neuronal development, synaptic maintenance and organization of neuronal networks. CNTNs are a subpopulation of molecules belonging to the immunoglobulin (Ig) superfamily, which includes six members: CNTN1, TAG-1/CNTN2, BIG-1/CNTN3, BIG-2/CNTN4, NB-2/CNTN5 and NB-3/CNTN61,2.In mammals, contactin-1 is mostly localized in the peripheral and central neural system. It is highly expressed at the axonal level and mediates various functions in both glial and neuronal cells; it is involved in neuronal and glial development and differentiation, myelination, synaptogenesis and synaptic function1.Contactin-1 expression profile differs in various brain regions and, within each area, it changes depending upon the cell type.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-NR171.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR171; Negative control: BLP-NR171.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
