| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prostaglandin G/H synthase 1;1.14.99.1;Cyclooxygenase-1;COX-1;Prostaglandin H2 synthase 1;PGH synthase 1;PGHS-1;PHS 1;Prostaglandin-endoperoxide synthase 1;PTGS1;COX1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human COX1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHF-16 is a rabbit monoclonal antibody that recognizes prostaglandin G/H synthase 1 (gene PTGS1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 68.7 kDa, and the supplier reports an observed band at about 70 kDa.
COX-1 (PTGS1) is a cyclooxygenase-peroxidase that turns arachidonic acid into prostaglandin H2, the common precursor of prostaglandins and thromboxanes. It is expressed constitutively in many tissues, including platelets and stomach lining, and is a target of aspirin and other NSAIDs.
| Target | Prostaglandin G/H synthase 1 (gene PTGS1; UniProt P23219, human) |
|---|---|
| Clone | FHF-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.7 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with confirmed staining (IHC): human uterus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human COX1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places prostaglandin G/H synthase 1 in the microsome membrane and endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jie Yi et al. (2022) Insights into the interaction of cyclooxygenase and lipoxygenase with natural compound 3,4′,5,7-Tetrahydroxyflavone based on multi-spectroscopic and metabolomics. Spectrochimica ACTA Part a-Molecular and Biomolecular Spectroscopy. 10.1016/j.saa.2022.121800
