| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR; DUSP3; VHR |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CPEB4 recombinant protein (Position: D3-N462). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CPEB4 Antibody Picoband® is an antibody for CPEB4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CPEB4 (dual specificity phosphatase 3); UniProt: Q17RY0
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 90 kDa, calculated 17828 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CPEB4 Antibody Picoband® catalog # A06139-2.
Biological background
Biological context: Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
Expression and localization notes: cellular localization: Nucleus., tissue context: Testis specific..
Common research applications
- Western blotting (WB): Compare CPEB4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CPEB4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CPEB4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Cytoplasmic polyadenylation element binding protein 4 (CPEB4) is a sequence-specific RNA-binding protein that participates in translational control. CPEB4 is a member of the CPEB family, which includes CPEB1, CPEB2, CPEB3, and CPEB4, all of which share structure and sequence identity in the C-terminal RNA-binding domain (RBD). CPEB4 has two domains: one that is structured for RNA binding and one that is unstructured and low complexity that has no known function.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Testis specific.
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
