| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytoplasmic polyadenylation element-binding protein 4; CPE-BP4; CPE-binding protein 4; hCPEB-4; CPEB4; KIAA1673 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CPEB4 recombinant protein (Position: D3-N462). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytoplasmic polyadenylation element-binding protein 4 (gene CPEB4) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 80.2 kDa, and the supplier reports an observed band at about 90 kDa.
CPEB4 is a cytoplasmic polyadenylation element-binding protein that regulates poly(A) tail length and translation of target mRNAs. It is involved in cell cycle progression, neuronal function and responses to cellular stress, and it is studied in translational control and cancer biology.
| Target | Cytoplasmic polyadenylation element-binding protein 4 (gene CPEB4; UniProt Q17RY0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 80.2 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells, SiHa cells.
Immunogen
Recombinant human CPEB4 fragment (Asp3–Asn462), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytoplasmic polyadenylation element-binding protein 4 in the cytoplasm, cell projection, postsynaptic density and endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in pancreas in islets and ductal cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
