| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cleavage and polyadenylation specificity factor subunit 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73; CPSF3; CPSF73 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CPSF73 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27C34 is a rabbit monoclonal antibody that recognizes cleavage and polyadenylation specificity factor subunit 3 (gene CPSF3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 77.5 kDa, and the supplier reports an observed band at about 77 kDa.
CPSF3 (also called CPSF73) is the endonuclease subunit of the cleavage and polyadenylation specificity factor complex. It cuts pre-mRNAs at the poly(A) site after recognition of the AAUAAA signal, enabling polyadenylation, and it also processes histone mRNAs, so it is studied in mRNA 3′-end formation.
| Target | Cleavage and polyadenylation specificity factor subunit 3 (gene CPSF3; UniProt Q9UKF6, human) |
|---|---|
| Clone | 27C34 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 77.5 kDa |
| Observed MW | 77 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human CPSF73.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cleavage and polyadenylation specificity factor subunit 3 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
