| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carnitine O-palmitoyltransferase 1, muscle isoform;CPT1-M;2.3.1.21;Carnitine O-palmitoyltransferase I, muscle isoform;CPT I;CPTI-M;Carnitine palmitoyltransferase 1B;Carnitine palmitoyltransferase I-like protein;CPT1B;KIAA1670; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CPT1B, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects carnitine O-palmitoyltransferase 1, muscle isoform (gene CPT1B) in mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 87.8 kDa, and the supplier reports an observed band at about 88 kDa.
Carnitine palmitoyltransferase 1B (CPT1B) is the muscle isoform of CPT1, expressed mainly in heart and skeletal muscle. It converts long-chain acyl-CoAs into acylcarnitines so that fatty acids can enter mitochondria for β-oxidation, and it is studied in cardiac and muscle energy metabolism.
| Target | Carnitine O-palmitoyltransferase 1, muscle isoform (gene CPT1B; UniProt Q92523, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 87.8 kDa |
| Observed MW | 88 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
Samples with a confirmed band (WB): rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse skeletal muscle tissue, rat cardiac muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): mouse cardiac muscle tissue, rat cardiac muscle tissue.
Samples with confirmed staining (ICC/IF): mouse cardiac tissue, rat cardiac tissue.
Immunogen
Synthetic peptide from the N-terminal region of human CPT1B. It differs from the mouse sequence at two residues and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places carnitine O-palmitoyltransferase 1, muscle isoform in the mitochondrion outer membrane. Tissue expression noted by UniProt (human): Strong expression in heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Daniel Da Eira et al. (2022) An obesogenic diet impairs uncoupled substrate oxidation and promotes whitening of the brown adipose tissue in rats. Journal of Physiology-London. 10.1113/JP283721
Yan Cao et al. (2021) Therapeutic evaluation and metabolic reprograming of isosteviol sodium in a rat model of ischemic cardiomyopathy. European Journal of Pharmacology. 10.1016/j.ejphar.2021.174539
Glen Katsnelson et al. (2020) Docosahexaenoic and eicosapentaenoic fatty acids differentially regulate glucose and fatty acid metabolism in L6 rat skeletal muscle cells. American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00304.2020
Xuebing Wang et al. (2024) miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats. Heliyon. 10.1016/j.heliyon.2024.e26572
Tang Bin et al. (2018) Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure. Bioscience Reports. 10.1042/BSR20171036
