Anti-CPT1B Antibody Picoband®

Overview

This antibody is intended for detection of CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-CPT1B Antibody Picoband® catalog # PB9491. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human CPT1B, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.
• Molecular weight context: reported MW: 88 kDa; calculated MW: 87801 MW
• Reactivity: Mouse,Rat
• Applications: IF, IHC, IHC-F, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Carnitine O-palmitoyltransferase 1, muscle isoform; Carnitine O-palmitoyltransferase 1, muscle isoform. CPT1B is located on 22q13.33. The protein encoded by this gene, a member of the carnitine/ choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. Functional note: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts ly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. . Reported localization: Mitochondrion outer membrane; Multi-pass membrane protein. Expression/tissue context: Strong expression in heart and skeletal muscle. No expression in liver and kidney.

Research relevance and current trends

• Cancer: Researchers commonly examine how CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) relates to this theme using model systems and orthogonal readouts.
• Cardiovascular: Researchers commonly examine how CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of CPT1B (Carnitine O-palmitoyltransferase 1, muscle isoform) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the carnitine/choline acetyltransferase family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.