| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carnitine O-palmitoyltransferase 2, mitochondrial;2.3.1.21;Carnitine palmitoyltransferase II;CPT II;CPT2;CPT1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CPT2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FGB-3 is a rabbit monoclonal antibody that recognizes carnitine O-palmitoyltransferase 2, mitochondrial (gene CPT2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 73.8 kDa, and the supplier reports an observed band at about 74 kDa.
Carnitine palmitoyltransferase 2 (CPT2) is an inner mitochondrial membrane enzyme that converts acylcarnitines back into acyl-CoAs inside the matrix. This allows long-chain fatty acids to undergo β-oxidation, and CPT2 deficiency is a common inherited disorder of fatty acid oxidation.
| Target | Carnitine O-palmitoyltransferase 2, mitochondrial (gene CPT2; UniProt P23786, human) |
|---|---|
| Clone | FGB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.8 kDa |
| Observed MW | 74 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human MCF-7 cells, human HEL cells, human RT4 cells, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human acinic cells carcinoma of parotid tissue, human cervical cancer tissue, human clear cells renal cells carcinoma tissue, human colon adenocarcinoma tissue, human liver cancer tissue, mouse heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Immunogen
Synthetic peptide based on the sequence of human CPT2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places carnitine O-palmitoyltransferase 2, mitochondrial in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
