| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinaldehyde-binding protein 1; Cellular retinaldehyde-binding protein; RLBP1; CRALBP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CRALBP Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEFB-18 is a rabbit monoclonal antibody that recognizes retinaldehyde-binding protein 1 (gene RLBP1) in mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 36.5 kDa, and the supplier reports an observed band at about 36 kDa.
Cellular retinaldehyde-binding protein (CRALBP, RLBP1) is a soluble retinoid carrier in the retinal pigment epithelium and Müller glia. It binds 11-cis-retinal and 11-cis-retinol and supports their regeneration in the visual cycle, which is needed for both rods and cones. RLBP1 mutations cause several inherited retinal dystrophies, and CRALBP is widely used as an RPE and Müller cell marker.
| Target | Retinaldehyde-binding protein 1 (gene RLBP1; UniProt P12271, human) |
|---|---|
| Clone | AEFB-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.5 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): mouse eyeball.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CRALBP.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places retinaldehyde-binding protein 1 in the cytoplasm. Tissue expression noted by UniProt (human): Retina and pineal gland. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
