| Field | Specification |
|---|---|
| Target | |
| Alternative names | CRBN; AD-006; Protein cereblon |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CRBN recombinant protein (Position: M1-H378). Human CRBN shares 95% amino acid (aa) sequence identity with both mouse and rat CRBN. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein cereblon (gene CRBN) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Cereblon (CRBN) is the substrate receptor of the CRL4CRBN E3 ubiquitin ligase, built with DDB1 and CUL4. Thalidomide, lenalidomide and pomalidomide bind cereblon and redirect it to degrade proteins such as IKZF1, IKZF3 and CK1α, so it is central to targeted protein degradation and PROTAC research.
| Target | Protein cereblon (gene CRBN; UniProt Q96SW2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human CRBN fragment (Met1–His378), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein cereblon in the cytoplasm, nucleus and membrane. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
