| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-directed RNA polymerase III subunit RPC9; RNA polymerase III subunit C9; Calcitonin gene-related peptide-receptor component protein; CGRP-RCP; CGRP-receptor component protein; CGRPRCP; HsC17; CRCP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CRCP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30C57 is a rabbit monoclonal antibody that recognizes DNA-directed RNA polymerase III subunit RPC9 (gene CRCP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 16.9 kDa, and the supplier reports an observed band at about 22 kDa.
CRCP (RPC9) is a subunit of RNA polymerase III, the enzyme that transcribes tRNAs, 5S rRNA and other small non-coding RNAs. It is also known as the CGRP receptor component protein, which couples the calcitonin gene-related peptide receptor to intracellular signaling.
| Target | DNA-directed RNA polymerase III subunit RPC9 (gene CRCP; UniProt O75575, human) |
|---|---|
| Clone | 30C57 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.9 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:2000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CRCP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-directed RNA polymerase III subunit RPC9 in the nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuekai Wang et al. (2026) Artesunate alleviates post-central stroke pain by inhibiting metabotropic glutamate receptor 5 in the cerebral cortex in rats. Neuroscience. 10.1016/j.neuroscience.2026.03.013
