| Field | Specification |
|---|---|
| Target | |
| Alternative names | CRH; Corticoliberin; Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CRF/CRH, identical to the related mouse and rat sequences. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets corticotropin-releasing hormone (gene CRH) in human, mouse and rat samples and is validated for Western blot and flow cytometry.
Corticotropin-releasing hormone (CRH) is a hypothalamic neuropeptide released in response to stress. Acting mainly through the CRHR1 receptor on pituitary corticotrophs, it stimulates ACTH secretion and thereby cortisol release, and it is a key subject of research on the hypothalamic–pituitary–adrenal axis and stress behavior.
| Target | Corticotropin-releasing hormone (gene CRH; UniProt P06850, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Immunogen
Synthetic peptide from the C-terminal region of human CRF/CRH. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes corticotropin-releasing hormone as a secreted protein. Tissue expression noted by UniProt (human): Produced by the hypothalamus and placenta.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
