| Field | Specification |
|---|---|
| Target | |
| Alternative names | CRISPR-associated endonuclease Cas9/Csn1 ;3.1.-.- ;SpCas9 ;SpyCas9 ;cas9 ;csn1 ;SPy_1046; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fragment derived from Streptococcus pyogenes. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABCI-3 is a rabbit monoclonal antibody that recognizes CRISPR-associated endonuclease Cas9/Csn1 in human and Recombinant protein samples and is validated for Western blot, IHC, ICC/IF and flow cytometry.
Cas9 is an RNA-guided DNA endonuclease from the type II CRISPR adaptive immune system of bacteria such as Streptococcus pyogenes and Staphylococcus aureus. Guided by a CRISPR RNA, it cuts double-stranded DNA next to a PAM sequence using its HNH and RuvC nuclease domains. Engineered Cas9 is the basis of CRISPR genome editing and is widely detected in edited cells and tissues.
| Target | CRISPR-associated endonuclease Cas9/Csn1 (gene cas9; UniProt Q99ZW2, Streptococcus pyogenes serotype M1) |
|---|---|
| Clone | ABCI-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Recombinant protein |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 158 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): 293T cells, 3T3 cells, PC12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (ICC/IF): 293T cells.
Immunogen
Recombinant fragment derived from Streptococcus pyogenes.
Reactivity Notes
The supplier lists reactivity with human and Recombinant protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ke Zhao et al. (2025) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic loci. Cell Genomics. 10.1016/j.xgen.2025.100982
Ziru Wang et al. (2023) Decreasing predictable DNA off-target effects and narrowing editing windows of adenine base editors by fusing human Rad18 protein variant. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2023.127418
