| Field | Specification |
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| Alternative Names | Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
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| Target |
Overview
Anti-CRLR/CALCRL (extracellular)-FITC Antibody is an antibody targeting Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR (also reported as Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR).
- Immunogen/epitope region: Extracellular, N-terminus..
- Homology note: Mouse, rat - 12 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ACR-060), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Fluorescein isothiocyanate (FITC) (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-F.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CR060; Negative control: RIC-001-F.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
