| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Gamma-sarcoglycan;Gamma-SG;35 kDa dystrophin-associated glycoprotein;35DAG;SGCG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CRMP4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DPYSL3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24D87; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-CRMP4 Rabbit Monoclonal Antibody catalog # M05960. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: DPYSL3 (Gamma-sarcoglycan).
- Antibody format: Monoclonal; clone 24D87; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DPYSL3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Reported cellular localization context: Cell membrane, sarcolemma ; Single-pass type II membrane protein . Cytoplasm, cytoskeleton . Tissue expression notes (as provided): Expressed in skeletal and heart muscle.
Research relevance and current trends
- Research context keywords from the source record include: Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate DPYSL3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DPYSL3 expression by Western blot in cell or tissue lysates, Localize DPYSL3 by immunofluorescence/immunocytochemistry in cultured cells, Enrich DPYSL3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 62 kDa; calculated MW: 32379 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 62 kDa
- Cellular localization (provided): Cell membrane, sarcolemma ; Single-pass type II membrane protein . Cytoplasm, cytoskeleton .
- Tissue details (provided): Expressed in skeletal and heart muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
