| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cold shock domain-containing protein E1; N-ras upstream gene protein; Protein UNR; CSDE1; D1S155E; KIAA0885; NRU; UNR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CSDE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30C76 is a rabbit monoclonal antibody that recognizes cold shock domain-containing protein E1 (gene CSDE1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 88.9 kDa, and the supplier reports an observed band at about 89 kDa.
CSDE1 (also called UNR) is a cytoplasmic RNA-binding protein with several cold shock domains. It helps couple translation with mRNA decay, for example of FOS mRNA, and is needed for efficient stress granule assembly. It also supports internal ribosome entry on some viral RNAs, such as human rhinovirus, so it is studied in post-transcriptional gene control and virology.
| Target | Cold shock domain-containing protein E1 (gene CSDE1; UniProt O75534, human) |
|---|---|
| Clone | 30C76 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.9 kDa |
| Observed MW | 89 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): K562 cells, PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CSDE1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cold shock domain-containing protein E1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
