| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CSK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24C62 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase CSK in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 50.7 kDa, and the supplier reports an observed band at about 50 kDa.
C-terminal Src kinase (CSK) is a non-receptor tyrosine kinase that switches off Src-family kinases such as SRC, LCK and FYN. It phosphorylates a tyrosine in their C-terminal tail, which folds the kinases into an inactive conformation. By limiting LCK and FYN, CSK sets the threshold for T-cell and B-cell receptor signaling and also affects cell growth and migration.
| Target | Tyrosine-protein kinase CSK (gene CSK; UniProt P41240, human) |
|---|---|
| Clone | 24C62 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.7 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CSK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase CSK in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in lung and macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
