| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-terminal-binding protein 1; CtBP1; CTBP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CtBP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20C16 is a rabbit monoclonal antibody that recognizes C-terminal-binding protein 1 (gene CTBP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 47.5 kDa, and the supplier reports an observed band at about 48 kDa.
CtBP1 is a transcriptional corepressor that binds many DNA-binding repressors, such as BCL6 and GLIS2, through a short PXDLS-type motif and recruits chromatin-modifying enzymes. It has an NAD(H)-dependent dehydrogenase domain that links its activity to cellular metabolic state, and it also affects Golgi membrane organization and brown fat development.
| Target | C-terminal-binding protein 1 (gene CTBP1; UniProt Q13363, human) |
|---|---|
| Clone | 20C16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.5 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ctBP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places C-terminal-binding protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in germinal center B-cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
