| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-terminal-binding protein 2;CtBP2;CTBP2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445). Human CTBP2 shares 99.2% and 98.4% amino acid (aa) sequence identity with mouse and rat CTBP2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CTBP2 (C-terminal-binding protein 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CTBP2 Antibody Picoband® catalog # PB9426. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CTBP2 recombinant protein (Position: H321-Q445). Human CTBP2 shares 99.2% and 98.4% amino acid (aa) sequence identity with mouse and rat CTBP2, respectively. (reported region: H321-Q445).
- Molecular weight context: reported MW: 50 kDa; calculated MW: 48945 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-terminal-binding protein 2; C-terminal-binding protein 2. The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1. Functional note: Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity). . Reported localization: Nucleus . Cell junction, synapse . Expression/tissue context: Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Research relevance and current trends
- Cell Type Marker: Researchers commonly examine how CTBP2 (C-terminal-binding protein 2) relates to this theme using model systems and orthogonal readouts.
- Co-Factors: Researchers commonly examine how CTBP2 (C-terminal-binding protein 2) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how CTBP2 (C-terminal-binding protein 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CTBP2 (C-terminal-binding protein 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CTBP2 (C-terminal-binding protein 2) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the cyclin family. Cyclin AB subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
