| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;CTLA-4;CD152;Ctla4;Cd152; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CTLA4 recombinant protein (Position: N110-N223). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytotoxic T-lymphocyte protein 4 (gene CTLA4) in human samples and is validated for IHC and ELISA. The predicted molecular weight is 24.7 kDa.
CTLA-4 (CD152) is an inhibitory receptor on activated T cells and regulatory T cells that dampens T-cell responses. It binds the B7 ligands CD80 and CD86 with much higher affinity than the costimulatory receptor CD28, competing with CD28 for these ligands and limiting activation. CTLA-4 is a key immune checkpoint studied in autoimmunity, tolerance and cancer immunotherapy.
| Target | Cytotoxic T-lymphocyte protein 4 (gene CTLA4; UniProt P16410, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 24.7 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human colon cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CTLA4 fragment (Asn110–Asn223), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cytotoxic T-lymphocyte protein 4 in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed with highest levels in lymphoid tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
