| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152; CTLA4; CD152 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CTLA4 (CD152) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADCD-3 is a rabbit monoclonal antibody that recognizes cytotoxic T-lymphocyte protein 4 (gene CTLA4) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 24.7 kDa, and the supplier reports an observed band at about 43 kDa.
CTLA-4 (CD152) is an inhibitory receptor on activated T cells and regulatory T cells that dampens T-cell responses. It binds the B7 ligands CD80 and CD86 with much higher affinity than the costimulatory receptor CD28, competing with CD28 for these ligands and limiting activation. CTLA-4 is a key immune checkpoint studied in autoimmunity, tolerance and cancer immunotherapy.
| Target | Cytotoxic T-lymphocyte protein 4 (gene CTLA4; UniProt P16410, human) |
|---|---|
| Clone | ADCD-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.7 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human CTLA4 (CD152).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytotoxic T-lymphocyte protein 4 in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed with highest levels in lymphoid tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiong Luojie et al. (2024) Immunopotentiating effects of herb-partitioned moxibustion on the spleens of cyclophosphamide-induced immunosuppressed rats. Chinese Medicine. 10.1186/s13020-024-00898-x
Zhan Zhuang et al. (2024) The Combination of Anti-CD47 Antibody with CTLA4 Blockade Enhances Anti-Tumor Immunity in Non-Small Cell Lung Cancer via Normalization of Tumor Vasculature and Reprogramming of the Immune Microenvironment. Cancers. 10.3390/cancers16040832
