| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cubilin;460 kDa receptor;Intestinal intrinsic factor receptor;Intrinsic factor-cobalamin receptor;Intrinsic factor-vitamin B12 receptor;CUBN;IFCR; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cubilin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GA-3 is a rabbit monoclonal antibody that recognizes cubilin (gene CUBN) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 398.7 kDa, and the supplier reports an observed band at about 399 kDa.
Cubilin (CUBN) is a large endocytic receptor in the kidney proximal tubule and small intestine that has no transmembrane domain of its own. With amnionless it takes up vitamin B12 bound to intrinsic factor in the gut, and with megalin (LRP2) it reclaims albumin and other filtered proteins in the kidney. It is studied in vitamin B12 absorption and renal protein handling.
| Target | Cubilin (gene CUBN; UniProt O60494, human) |
|---|---|
| Clone | GA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 398.7 kDa |
| Observed MW | 399 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human cubilin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cubilin in the apical cell membrane, cell membrane, membrane and endosome. Tissue expression noted by UniProt (human): Detected in kidney cortex (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
