| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | G2/mitotic-specific cyclin-B1;CCNB1;CCNB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Cullin 3 Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CUL3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEOD-3; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP, Flow (as provided in the source record). Boster Bio Anti-Cullin 3 Monoclonal Antibody catalog # M00747. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CUL3 (G2/mitotic-specific cyclin-B1).
- Antibody format: Monoclonal; clone AEOD-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CUL3 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Essential for the control of the cell cycle at the G2/M (mitosis) transition. . Reported cellular localization context: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Tissue expression notes (as provided): Ubiquitous. Expressed at high levels in testis.
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Cancer,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Cell Death,Nucleus,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,SCF Complex E3 Ligase,Ubiquitin E3 Enzymes.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CUL3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CUL3 expression by Western blot in cell or tissue lysates, Quantify CUL3-positive cells by flow cytometry in single-cell suspensions, Enrich CUL3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 133 kDa; calculated MW: 48337 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 133 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
- Tissue details (provided): Ubiquitous. Expressed at high levels in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
