| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cullin-5; CUL-5; Vasopressin-activated calcium-mobilizing receptor 1; VACM-1; CUL5; VACM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Cullin 5 recombinant protein (Position: A2-D105). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cullin-5 (gene CUL5) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 91.0 kDa, and the supplier reports an observed band at about 91 kDa.
Cullin-5 (CUL5) is the scaffold of CRL5 E3 ubiquitin ligases, which pair with Elongin B/C, the RING protein RBX2 and SOCS-box substrate receptors. These ligases regulate cytokine signaling through SOCS proteins and other targets, and HIV-1 Vif hijacks CUL5 to degrade the antiviral APOBEC3 proteins. It is studied in signaling, protein turnover and virology.
| Target | Cullin-5 (gene CUL5; UniProt Q93034, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 91.0 kDa |
| Observed MW | 91 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat spleen tissue, rat heart tissue, rat kidney tissue, mouse brain tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human Cullin 5 fragment (Ala2–Asp105), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cullin-5 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
