| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 11; Beta-R1; H174; Interferon gamma-inducible protein 9; IP-9; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11; CXCL11; ITAC; SCYB11; SCYB9B |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CXCL11 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18C54 is a rabbit monoclonal antibody that recognizes C-X-C motif chemokine 11 (gene CXCL11) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 10.4 kDa, and the supplier reports an observed band at about 15 kDa.
CXCL11 (I-TAC) is an interferon-inducible chemokine that binds the receptor CXCR3, and also ACKR3, with high affinity. It attracts activated T cells, but not resting T cells, neutrophils or monocytes, and triggers calcium release in them. It is studied alongside CXCL9 and CXCL10 in Th1-type inflammation, the central nervous system and skin immunity.
| Target | C-X-C motif chemokine 11 (gene CXCL11; UniProt O14625, human) |
|---|---|
| Clone | 18C54 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.4 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human THP-1 cells, human SH-SY5Y cells, human U251 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CXCL11.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-X-C motif chemokine 11 as a secreted protein. Tissue expression noted by UniProt (human): High levels in peripheral blood leukocytes, pancreas and liver astrocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
