| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; SDF-1-beta (3-72); SDF-1-alpha (3-67); CXCL12; SDF1, SDF1A, SDF1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse CXCL12, which shares 96.2% and 95.5% amino acid (aa) sequence identity with human and rat CXCL12, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects stromal cell-derived factor 1 (gene CXCL12) in mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 10.7 kDa, and the supplier reports an observed band at about 11 kDa.
CXCL12 (SDF-1) is a chemokine that binds the receptors CXCR4 and ACKR3. It retains hematopoietic stem cells in the bone marrow niche, guides lymphocyte and monocyte migration, and directs cell movement during embryonic development, including neural, vascular and germ cell migration. It is also studied in cancer metastasis and tissue repair.
| Target | Stromal cell-derived factor 1 (gene CXCL12; UniProt P48061, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 10.7 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
Samples with a confirmed band (WB): rat stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of mouse CXCL12. Sequence identity with the human and rat orthologs: 96.2% and 95.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes stromal cell-derived factor 1 as a secreted protein. Tissue expression noted by UniProt (human): Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qingxia Hu et al. (2018) Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration. ACS Applied Materials & Interfaces. 10.1021/acsami.8b08668
Zong Zhao-Wen et al. (2006) Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow. Journal of Radiation Research. 10.1269/jrr.0531
