| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 1;CXC-R1;CXCR-1;CDw128a;High affinity interleukin-8 receptor A;IL-8R A;IL-8 receptor type 1;CD181;CXCR1;CMKAR1, IL8RA; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C chemokine receptor type 1 (gene CXCR1) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 39.8 kDa, and the supplier reports an observed band at about 50 kDa.
CXCR1 is a G protein-coupled receptor for interleukin-8 (CXCL8) and the related chemokine CXCL6. It is highly expressed on neutrophils, where binding of IL-8 triggers calcium signaling, chemotaxis, degranulation and oxidative burst. It is studied with CXCR2 in neutrophil recruitment, inflammation and cancer.
| Target | C-X-C chemokine receptor type 1 (gene CXCR1; UniProt P25024, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 39.8 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): human PBMC cells.
Immunogen
Synthetic peptide from the N-terminal region of human CXCR1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places C-X-C chemokine receptor type 1 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Guangchen et al. (2019) Activation of CXCL6/CXCR1/2 Axis Promotes the Growth and Metastasis of Osteosarcoma Cells in vitro and in vivo. Frontiers in Pharmacology. 10.3389/fphar.2019.00307
